Antibacterial Activity of Stylissa carteri Sponge Extract from Manado Bay, North Sulawesi

Marine sponges contain secondary metabolites with unique chemical structures and very interesting pharmacological activities, such as antibacterial, anticancer, antiviral others to be developed candidate drugs. The presence of bioactive in sessile nature also reflects the ecological adaptation formed during a long evolutionary process defense mechanism this organism its environment form resistance predation, competition infection factors against pathogenic bacteria, so study aims examine antibacterial activity extracts fractions Stylissa carteri sponge well testing Minimum Inhibitory Concentration (MIC) Bactericidal (MBC) values. method used is agar diffusion (Disc Diffusion Kirby Bauer Method). was indicated by formation clear zone around paper disc after incubation for 24 hours. results showed that S. extract tested on Bacillus megaterium DSM32T bacteria revealed strongest inhibition 21 mm. Further fraction semipolar strong B. while polar categorized moderate action, non-polar no bacteria. determination MIC MBC values obtained at 500 ppm 1000 respectively.